PL aquagel-OH Applications

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Polyethylene Glycol / Oxide Standards

Polyethylene Glycol / Oxide Standards (PL aquagel-OH)

Column:
PL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: Water
Flow Rate: 1.0ml/min
Detector: RI

Standards
1. 1,702,000
2. 120,000
3. 12,600
4. 1,470
5. 106

Polystyrene Sulfonate Standards (anionic, hydrophobic)

Polystyrene Sulfonate Standards (PL aquagel-OH)

Columns:
3xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 70% 0.2M NaNO3, 0.01 NaH2PO4
pH 9, 30% Methanol
Flow Rate: 1.0ml/min
Detector: RI

Standards
1. 400,000
2. 35,000
3. 4,600

Pullulan Polysaccharide Standards

Pullulan Polysaccharide Standards (PL aquagel-OH)

Columns:
3xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Standards
1. 788,000
2. 212,000
3. 47,300
4. 11,800
5. 667

Chitosan Samples (cationic)

Chitosan Samples (PL aquagel-OH)

Inbalance peaks on RI as samples are prepared in strong acid for dissolution.

Columns:
2xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.5M NaNO3, 0.01 NaH2PO4, pH 2
Flow Rate: 1.0ml/min
Detector: RI

Pectin

Pectin (PL aquagel-OH)

Columns:
3xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Polyvinyl Alcohol (98% hydrolyzed)

Polyvinyl Alcohol (PL aquagel-OH)

Columns:
3xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Dextran

Dextran (PL aquagel-OH)

Columns:
2xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Sodium Alginate

Sodium Alginate (PL aquagel-OH)

Columns:
2xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Polyethylene Oxide Standards

Polyacrylic Acid (PL aquagel-OH)

Overlaid separations obtained from the SEC of PEO EasiVials under the SEC conditions described opposite.

Columns:
2xPL aquagel-OH MIXED-H 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, adjusted to pH 7
Flow Rate: 1.0ml/min
Inj Vol: 100µl
Sample Conc: PEO standards:0.1 - 0.5mg/ml, Polyacrylic acid: approx 0.2% w/v
Detector: RI

Polyethylene Glycol Standards

Polyethylene Glycol Standards (PL aquagel-OH)

Columns:
2xPL aquagel-OH 30 8µm, 300x7.5mm
Eluent: Water
Flow Rate: 1.0ml/min
Detector: RI

KEY
1. 12,600
2. 4,120
3. 1,470
4. 440
5. 106

Polysaccharide Standards

Polysaccharide Standards (PL aquagel-OH)

Columns:
2xPL aquagel-OH 30 8µm, 300x7.5mm
Eluent: Water
Flow Rate: 1.0ml/min
Detector: RI

Standards
1. 23,700
2. 5, 800
3. 667
4. 180

Heparin

Heparin (PL aquagel-OH)

Columns:
2xPL aquagel-OH 30 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Non-ionic Surfactant (Pluronic F68) (hydrophobic)

Non-ionic Surfactant (PL aquagel-OH)

Columns:
2xPL aquagel-OH 30 8µm, 300x7.5mm
Eluent: 70% 0.2M NaNO3, 0.01 NaH2PO4,
pH 7, 30% Methanol
Flow Rate: 1.0ml/min
Detector: RI

Polyacrylic Acid

Mw 960,000
Polyacrylic Acid (PL aquagel-OH)

Columns:
PL aquagel-OH 60 15µm, 300x7.5mm
PL aquagel-OH 40 15µm, 300x7.5mm Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 0.5ml/min
Detector: RI

Guar Gum

Guar Gum (PL aquagel-OH)

Columns:
PL aquagel-OH 60 15µm, 300x7.5mm
PL aquagel-OH 40 15µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 0.5ml/min
Detector: RI / LS 15° / LS 90°

Hyaluronic Acid

Hyaluronic Acid (PL aquagel-OH)

Columns:
PL aquagel-OH 60 15µm, 300x7.5mm
PL aquagel-OH 40 15µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Polyacrylamide

Polyacrylamide (PL aquagel-OH)

Columns:
PL aquagel-OH 60 15µm, 300x7.5mm
PL aquagel-OH 40 15µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Sodium Polyacrylate

Sodium Polyacrylate (PL aquagel-OH)

Columns:
PL aquagel-OH 40 8µm, 300x7.5mm
PL aquagel-OH 30 8µm, 300x7.5mm Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Residual Polyacrylamide in Reclaimed Water

Residual Polyacrylamide in Reclaimed Water (PL aquagel-OH)

Columns:
PL aquagel-OH 40 8µm, 300x7.5mm
PL aquagel-OH 30 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Dextrin Sulfate

Dextrin Sulfate (PL aquagel-OH)

Columns:
PL aquagel-OH 40 8µm, 300x7.5mm
PL aquagel-OH 30 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI

Dextran T40

Dextran T40 (PL aquagel-OH)

Columns:
PL aquagel-OH 40 8µm, 300x7.5mm
PL aquagel-OH 30 8µm, 300x7.5mm
Eluent: 0.2M NaNO3, 0.01 NaH2PO4, pH 7
Flow Rate: 1.0ml/min
Detector: RI