Efficient nebulization using low gas flow rates is a feature of PL's detectors. Independent nebulizer temperature control and digital gas flow control provide excellent stability and reproducibility. Baseline noise is minimized by the removal of any poorly nebulized eluent via a drain port.
The nebulized stream passes through an independently temperature-controlled evaporator tube where the solvent is removed, leaving the less volatile solute particles. PL's detectors feature the shortest evaporator tubes which guarantee extremely low swept volume and minimum peak dispersion. The result is that PL's detectors deliver you the maximum resolution from your separation, especially important for work with small column volumes.
Resolution of phospholipids can be difficult to achieve without using a complex gradient. PL's evaporative light scattering detectors can efficiently evaporate even complex gradients, therefore proving ideal for the detection of polar lipids. This chromatogram shows the stability of PL's ELSDs for the detection of phospholipids.
Sample: Polar Lipid mix at a concentration of 3.125 mg/mL
Column: S5 Spherisorb, 100x4.6mm
Eluent A: 99% 2,4,4-Trimethylpentane : 1% THF
Eluent B: 66.6% Acetone : 33.3% DCM
Eluent C: 15% Water : 85% Isopropanol, 7.5mM Ethanolamine, 7.5mM Acetic acid
Gradient: See table
Flow Rate: See table
KEY
1. Cholesterol
2. Phosphatidylserine
3. Phosphatidylethanolamine
4. Phosphatidylcholine
| Time | % Solvent | Flow Rate | ||
| Min | A | B | C | mL/min |
| 0 | 100 | - | - | 1.6 |
| 4 | 90 | 10 | - | 1.6 |
| 7 | 70 | 30 | - | 1.6 |
| 7.1 | 40 | 50 | 10 | 1.6 |
| 20.4 | 39 | - | 61 | 1.6 |
| 20.5 | 40 | 60 | - | 2 |
| 22.6 | 100 | - | - | 2 |
| 25 | 100 | - | - | 2 |
| 25.1 | 100 | - | - | 2 |
| 30 | 100 | - | - | 2 |
The solute particles are detected as they pass through the optical chamber. The advanced design of the optical and electronic components ensures that PL's detectors deliver maximum sensitivity with minimum baseline noise.