Biomagnetic separation today is a mainstream technology in life science research, clinical diagnostics and therapeutics. With so many new and demanding applications, it is vital that research scientists, product developers, manufacturers and healthcare professionals have access to top quality paramagnetic particle products which they can rely on for performance and consistency.
LodeStars, coupled with the chosen biomolecule, are allowed to bind and form a complex with the target. For example, LodeStars 2.7 Streptavidin will bind with a biotinylated ligand. After a short reaction time, a magnetic field is applied with a PL-MCS Magnetic Capture Stand, and the LodeStars recovered from the sample solution. The separation is fast, efficient and gentle. It requires no columns or centrifugation. Washing and handling using magnetic particles lends itself to automated processing that is quick and high reproducible.
LodeStars' polymer surface is highly controlled to provide ultra-low non-specific binding of unwanted sample components and to reduce non-covalent biomolecule attachment that might cause loss of biomolecules in storage or during a procedure. LodeStars are highly resistant to mechanical stress and stable over a wide pH range and reducing conditions.
Diameter |
2.7µm |
c.v. |
3% |
Concentration |
30mg/ml |
Bead Numbers |
2.5x109/ml |
Iron Content |
20% |
Surface Area |
>2m2/g |
Magnetic Mass Susceptibility |
>60m3/kg |
Surface Chemistry |
Carboxylic acid |
Antibody Coupling Capacity |
up to 10µg/mg |
Based on PL's patented technology, LodeStars products have applications across numerous areas of bioscience research, diagnostics and therapeutics, as well as the development of new products in molecular medicine.
Polymer Laboratories is one of the world's largest producers of particles for bioseparations. PL is ISO 9001:2000 accredited, with nearly 30 years' experience in bead manufacture and applications development. Our technologies are widely used in chromatography, diagnostics and pharmaceutical chemistries, and our beads are manufactured according to patented processes owned by, or licensed to, Polymer Laboratories.
LodeStars are manufactured under stringent controls to ensure batch to batch reproducibility of physical and chemical properties.
| Product | Immunoglobulin Binding µg/mg Beads | ||
| Affinity purified polyclonal goat anti-mouse IgG (Fc) |
Immunocapture of mouse IgG |
Performance Efficiency µg mouse IgG/µg goat anti-mouse IgG | |
| LodeStars 2.7 Carboxyl |
9µg/mg | 4.0µg/mg | 0.44 |
| Competitor's 2.7 Carboxyl equivalent |
11µg/mg | 2.8µg/mg | 0.25 |
In these experiments, LodeStars 2.7 Carboxyl consumed less goat-anti mouse first antibody and captured significantly more mouse IgG than the leading competitor. The LodeStars' surface allows high functional availability of bound ligand leading to better all round performance metrics.
| Product | Biotin binding capacity pmole/mg (Oregon Green® 488 - desthiobiocytin conjugate) |
Biotinylated antibody binding capacity µg/mg (Biotinylated IgG goat anti-fluorescein) |
| LodeStars 2.7 Streptavidin |
>900 | 6.3 |
| Competitor's 2.7 Streptavidin equivalent |
>800 | 3.0 |
In each case, LodeStars 2.7 Streptavidin showed improved performance metrics.
LodeStars are manufactured under stringent controls to ensure batch to batch reproducibility of physical and chemical properties.